Urinary tract infections magdalena sobieszczyk, md mph division of infectious diseases columbia university. Between 2005 and 2009, a total of 57 cases of peritonsillar. Ppt the gram stain powerpoint presentation free to. Beyer individual supplies microscope slide clothes pin inoculating loop or wood splint paper towels shared supplies crystal violet stain safranin red stain grams iodine ethyl alcohol denatured water bottle rinsing basin alcohol burner wax pencil matches microscope create a staining area on your microscope slide obtain a clean microscope slide draw a.
Alternatively, flood smear with methanol for 1 minute. Scribd is the worlds largest social reading and publishing site. Gram stain is probably one of the most commonly used staining procedures used in the field of microbiology. Classic gram staining techniques involve the following steps. Gram and acidfast stain control slides elitechgroup.
Please note that the quality of the smear too heavy or too light cell concentration will affect the gram stain results. Bacteria gram stain give what you know about genetics and molecular biology, describe another way you might be able to identify specific bacteria. Generally, an ebook can be downloaded in five minutes or less. Gram staining is based on the ability of the bacterial cell wall to retain the crystal violet dye. Ppt gram stain procedure powerpoint presentation free. To examine whether gram staining can influence the choice of antibiotic for the treatment of peritonsillar abscess.
View and download powerpoint presentations on spore stain ppt. Principle, procedure and results february 2, 2015 acharya tankeshwar bacteriology, staining techniques in microbiology 45 gram staining method, the most important procedure in microbiology, was developed by danish physician hans christian gram in 1884. The staining technique, developed in the late 1700s by christian gram classifies the rigid cell walled bacteria into one of two groups based on whether they are able to resist the decolorizing action of an alcoholic. Different bacteria stain differently to a common staining procedure. Staphylococcus epidermidis a gram positive cocci found on the. How can you tell a bacterial cell from normal body cells when a possible infection has taken place slideshow 3147360 by dyllis.
Microscopy and staining chapter 3 metric units light properties wavelength polarity waves can be added light properties resolution wavelengthresolution interaction light properties reflection transmission light properties absorption refraction light microscopy types compound bright field oil immersion microscopy dark field microscopy phase contrast dual beam phase contrast phase. Oral microbiology bacteria causing inflammations and abscesses. View and download powerpoint presentations on capsule staining ppt. Stain removal ppt free download as powerpoint presentation. Add the primary stain crystal violet and incubate 1 minute. Apr 04, 2016 a staining technique used to classify bacteria. Bacteria may be stained by a variety of dyes, including. Gonorrhea centers for disease control and prevention. Gram stain of urine shows numerous gram negative rods. If so, share your ppt presentation slides online with. The gram stain of a bacteria is determined by one thing gram positive gram negative the thickness of the petidoglycan in its cell wall materials background clothes pin 2. The gram stain control slides are prepared from standard quality control organisms.
Some counterstains which are acidic may lighten or remove the nuclear stains. It was a few years later, that the german pathologist carl weigert18451904 from frankfurt, added a final step of staining with safranin. Gram staining is an essential procedure used in the identification of bacteria and is frequently the only method required for studying their morphology. Find powerpoint presentations and slides using the power of, find free presentations research about spore stain ppt. Selection of empirical antibiotics based on gram stain finding. Powerpoint presentation on gauchers disease or powerpoint presentation on wrightgiemsa stained bone marrow aspirate shows a gauchers cell containing abundant granular or fibrillary, bluegray cytoplasm with. Gram staining is a differential staining technique that provides an easy differentiation of bacteria into one of two groups. The gram stain reaction, named after the danish bacteriologist hans christian gram, was originally devised in 1882. On this side draw a circle in the clear section of the slide. The two most important methods, the gram and acidfast techniques, use staining, decolorization, and counterstaining in a manner that helps to classify as well as stain the organism.
How it works gram positive bacteria have a thick layer ofpeptidoglycanup to 90%in their cell wall gram negative bacteria have a thinner layer 5 20%of this glycoprotein. Fix the dried film by passing it briefly through a bunsen flame. The gram stain, the most widely used staining procedure in bacteriology, is a complex. View and download powerpoint presentations on gram positive cocci ppt. Selection of suitable culture media based on gram stain finding. Free download viruses powerpoint presentation slides free.
The gram stain procedure was originally developed by the danish physician hans. Differential stain distinguishes between different groups of bacteria based on their staining ability. State why the gram stain is said to be a differential stain describe the differences between a gram positive and a gram negative cell wall state the mechanism of the gram stain and why differential staining of. It includes introduction, history,principle, method of gram staining procedure slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. The gram stain reaction and koh test results were compared with the antibiotic disk susceptibilities vancomycin and colistin the preliminary.
Rapid presumptive diagnosis of diseases such as bacterial meningitis. The gram stain is the property of its rightful owner. These online assessment tools will help you strengthen your understanding of the gram stain method. The difference in result is due to the differences in the cell wall structure or composition of bacteria that causes the different in the reaction with the series of reagents in gram staining talaro, 2007. Fixation of clinical materials to the surface of the microscope slide either by. He developed a new method to stain bacteria so they can be visible in specimen samples.
Ppt the gram stain powerpoint presentation free to download id. Record sketches of the organisms, size, color, morphology, and culture identification. Gram staining free download as powerpoint presentation. Powerpoint is the worlds most popular presentation software which can let you create professional bacteria are everywhere powerpoint presentation easily and in no time. Gram negative bacteria release the first dye used and appear red from the second counter dye. The ones who study about this is the ones in genetic and biology field. Actinomyces israelii gram stain gram positive bacteria that form long, branching filaments resembling the fungi 26. Differential stain hans christian gram, a danish doctor. Flood airdried, heatfixed smear of cells for 1 minute with crystal violet staining reagent. Who developed gram stains hans christian gram danish physician 1884 original purpose was to discern the difference between two types of pnuemonia. Gram staining of bacteria the purpose of gram staining is to determine one characteristic property of bacteria. Biology ppts videos microbiology mcqs microbiology notes. The adobe flash plugin is needed to view this content. To help you more easily find what youre looking for, select the see more link of the subtopic below that corresponds to your interests or use the search boxes.
Make a slide of tissue or body fluid that is to be stained. Who developed gram stains hans christian gram danish physician 1884 original purpose was to discern the difference. Heat the slide for few seconds until it becomes hot to the touch so that bacteria are firmly mounted to the slide. Gram staining of bacterial cells is neither an accurate nor elaborate technique, but nevertheless it is practically useful to distinguish two big domains of eubacteria species. Developed gram staining technique which is unique to bacterial cells due to their cell wall construction. Gram himself never used the red counterstaining in order to visualize the gram. Learn for free about math, art, computer programming, economics, physics, chemistry, biology, medicine, finance, history, and more. Gram staining a important technique a staining technique used to classify bacteria. Gonorrhea gonococcal urethritis gonococcal cervicitis gonorrhea gram stain of urethral discharge bartholins abscess bartholins abscess gonococcal ophthalmia disseminated gonorrhea skin lesion disseminated gonorrhea skin lesion gonorrhea gonococcal urethritis gonococcal cervicitis gonorrhea gram stain of urethral discharge bartholins abscess bartholins abscess gonococcal. It is one of the differential stains that are used to characterize bacteria in one of two groups. This site uses cookies to deliver our services and to show you relevant ads and presentations.
Traditional definition of gram stain a method of staining bacteria using a violet stain. Overheating of smears when heat fixation is used d. Gram stain was described by danish bacteriologist hans christian gram in 1884 to differentiate streptococcus pneumoniae from klebsiella pneumoniae in lung tissue. In the laboratory, the gram stain test is the primary test employed in ppt. Beyer individual supplies microscope slide clothes pin inoculating loop or wood splint paper towels shared supplies crystal violet stain safranin red stain gram s iodine ethyl alcohol denatured water bottle rinsing basin alcohol burner wax pencil matches microscope create a staining area on your microscope slide obtain a clean microscope slide draw a. Bacterial classification, structure and function introduction the purpose of this lecture is to introduce you to terminology used in microbiology. Times new roman arial wingdings default design slide 1 key words prokaryotes prokaryotes bacteria eukaryotes prokaryotic cell versus eukaryotic cell bacteria versus archaebacteria bacteria versus archaebacteria slide 9 bacteria slide 11 slide 12 slide outer membrane gram negative cell envelope gram positive cell envelope flagella slide. Ppt gram staining powerpoint presentation, free download. Find powerpoint presentations and slides using the power of, find free presentations research about capsule staining ppt.
You will be asked about differential staining, gram positive and. Gram staining or gram s method is an emprical method of differentiating bacterial species into two large groups gram positive and gram negative based on the chemical and physical properties of their cell wall. Download gram positive bacteria 319476 ppt for free. Without it, dye is not capable of binding to and staining the tissue. View and download powerpoint presentations on staining techniques ppt. Screening of the quality of the clinical specimens such as sputum that should contain many. Label the frosted side of your slide with your initials, the name of the organism, and the date. Gram positive bacteria that include most of the firmicutes, and gram negative bacteria that include the rest.
Find powerpoint presentations and slides using the power of, find free presentations research about staining techniques ppt. Crystal violet primary stain for 1 minrinse gram s iodine mordant for 1 minrinse acetonealcohol decolorizer for 5 seconds safranin counterstain for 1 minrinse gram negative red and gram positive blue assess gram reaction a. In the first step, the nuclei are stained with a hematoxylin solution. The gram stain is almost always the first step in the identification of a bacterial organism, while gram staining is a valuable. Basic microbiology lab reporting gram staining free download as powerpoint presentation. Gram stain this is the most important staining method in bacteriology. A free powerpoint ppt presentation displayed as a flash slide show on id. In addition this stain also allows determination of cell morphology, size, and arrangement. View and download powerpoint presentations on gram staining ppt. Ppt gram staining powerpoint presentation free to download id. Mordants substance that causes certain staining reactions to take place by forming a link between the tissue and the stain. Cover different classification schemes for grouping bacteria, especially the use of the gram stain 2. Jun 10, 2006 such staining methods are called differential staining methods, these include gram staining and acid fast staining. Principle, procedure, interpretation, examples and animation last updated.
Gram staining is a differential staining technique that differentiates bacteria into two groups. Find powerpoint presentations and slides using the power of, find free presentations research about gram staining ppt. Knowing gram positive or gram negative helps prescribe appropriate antibiotic. Ppt giemsa powerpoint slides power point presentations. Gram staining is the property of its rightful owner. Our gram stain control slides have two wells containing a gram positive control organisms staph. The most widely used staining procedure in microbiology is the gram stain, discovered by the danish scientist and physician hans christian joachim gram in 1884. Differentiate bacteria into two large groups the gram positive and the gram negative. Gram staining lab 3 hans christian gram developed gram staining in 1844 the reagents primary stain.
Christian gram in 1884, and categorizes bacteria according to their gram character gram positive or gram negative. Basic microbiology lab reporting gram staining staining. Find powerpoint presentations and slides using the power of, find free presentations research about gram positive cocci ppt. Gram staining 2 free download as powerpoint presentation. Staining, therefore, is of primary importance for the. They study the structure and function of gene at a molecular. Introduction as bacteria consist of clear protoplasmic matter, differing but slightly in refractive index from the medium in which they are growing, it is difficult with the ordinary microscope, except when special methods of illumination are used, to set them in the unstained condition. They either take stain very well gram positive and are purple in color, or they are dont take stain very well gram negative and are pink in color. This helps you give your presentation on bacteria are everywhere in a conference, a school lecture, a business proposal, in a webinar and business and professional representations the uploader spent hisher valuable time to. How to prepare a bacterial smear for gram staining the microbiology image library is the largest photo collection on the spo site. To be visible on a slide, organisms that stain by the gram method must be. Fix the smear by passing it over the flame 23 times.
Ppt gram stain powerpoint presentation, free download. Gram stain mohammad rahbar reporting 4 issue a written preliminary report with the gram s stain results 5 telephonically notify the ordering provider or ward of. June 12, 2018 by sagar aryal gram staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by danish bacteriologist hans christian gram in. The microscopicanalysis using gram staining technique showed that majority of the bacterial isolates were grampositive bacteria while only three of. Gramstain mohammad rahbar reporting 4 issue a written preliminary report with the gram s stain results 5 telephonically notify the ordering provider or ward of. Gram stain control slide provides two airdried and methanolfixed droplets within two etched circles. Gram staining chelsea bianchi how does gram staing work.
Learning how to perform a gram stain will be the starting step in determining where to start when identifying bacteria for illnesses, disease or infection. Bacterial characteristics gram staining video khan academy. Gram stain separates almost all bacteria in to 2 large groups gram positive bacteria that stain blue thick cell wall layer gram negative bacteria that stain pink thin cell wall layer the gram. Ppt gramstain powerpoint presentation free to download.
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