Urinary tract infections magdalena sobieszczyk, md mph division of infectious diseases columbia university. Between 2005 and 2009, a total of 57 cases of peritonsillar. Gram stain separates almost all bacteria in to 2 large groups gram positive bacteria that stain blue thick cell wall layer gram negative bacteria that stain pink thin cell wall layer the gram. Heat the slide for few seconds until it becomes hot to the touch so that bacteria are firmly mounted to the slide. Gram stain of urine shows numerous gram negative rods. Gram and acidfast stain control slides elitechgroup. Jun 10, 2006 such staining methods are called differential staining methods, these include gram staining and acid fast staining. To be visible on a slide, organisms that stain by the gram method must be. View and download powerpoint presentations on spore stain ppt. Traditional definition of gram stain a method of staining bacteria using a violet stain. Methylene blue, crystal violet, carbolfuchsin red, and safranin red. The ones who study about this is the ones in genetic and biology field. June 12, 2018 by sagar aryal gram staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by danish bacteriologist hans christian gram in. Gram staining is based on the ability of the bacterial cell wall to retain the crystal violet dye.
Alternatively, flood smear with methanol for 1 minute. How can you tell a bacterial cell from normal body cells when a possible infection has taken place slideshow 3147360 by dyllis. Bacterial characteristics gram staining video khan academy. To help you more easily find what youre looking for, select the see more link of the subtopic below that corresponds to your interests or use the search boxes. Basic microbiology lab reporting gram staining staining. Add the primary stain crystal violet and incubate 1 minute. Our gram stain control slides have two wells containing a gram positive control organisms staph. Gram stain was described by danish bacteriologist hans christian gram in 1884 to differentiate streptococcus pneumoniae from klebsiella pneumoniae in lung tissue. Some counterstains which are acidic may lighten or remove the nuclear stains. You will be asked about differential staining, gram positive and.
Rapid presumptive diagnosis of diseases such as bacterial meningitis. Apr 04, 2016 a staining technique used to classify bacteria. Gram staining is a simple procedure if done with precision and accuracy can guide in diagnosis of several bacterial infections. Staining techniques in microbiology a presentation by g. On this side draw a circle in the clear section of the slide. Selection of empirical antibiotics based on gram stain finding. The two most important methods, the gram and acidfast techniques, use staining, decolorization, and counterstaining in a manner that helps to classify as well as stain the organism. These online assessment tools will help you strengthen your understanding of the gram stain method. Please note that the quality of the smear too heavy or too light cell concentration will affect the gram stain results. How to prepare a bacterial smear for gram staining the microbiology image library is the largest photo collection on the spo site. Beyer individual supplies microscope slide clothes pin inoculating loop or wood splint paper towels shared supplies crystal violet stain safranin red stain gram s iodine ethyl alcohol denatured water bottle rinsing basin alcohol burner wax pencil matches microscope create a staining area on your microscope slide obtain a clean microscope slide draw a.
They either take stain very well gram positive and are purple in color, or they are dont take stain very well gram negative and are pink in color. It was a few years later, that the german pathologist carl weigert18451904 from frankfurt, added a final step of staining with safranin. It is one of the differential stains that are used to characterize bacteria in one of two groups. Christian gram in 1884, and categorizes bacteria according to their gram character gram positive or gram negative. View and download powerpoint presentations on gram staining ppt. Selection of suitable culture media based on gram stain finding. Fix the smear by passing it over the flame 23 times. Gram staining is an essential procedure used in the identification of bacteria and is frequently the only method required for studying their morphology. Make a slide of tissue or body fluid that is to be stained. Storing slides in a jar with 95% ethanol will ensure clean slides.
They study the structure and function of gene at a molecular. Powerpoint presentation on gauchers disease or powerpoint presentation on wrightgiemsa stained bone marrow aspirate shows a gauchers cell containing abundant granular or fibrillary, bluegray cytoplasm with. If so, share your ppt presentation slides online with. Find powerpoint presentations and slides using the power of, find free presentations research about staining techniques ppt. Knowing gram positive or gram negative helps prescribe appropriate antibiotic. Fixation of clinical materials to the surface of the microscope slide either by. Gramstain mohammad rahbar reporting 4 issue a written preliminary report with the gram s stain results 5 telephonically notify the ordering provider or ward of. Gram stain is probably one of the most commonly used staining procedures used in the field of microbiology. Microscopy and staining chapter 3 metric units light properties wavelength polarity waves can be added light properties resolution wavelengthresolution interaction light properties reflection transmission light properties absorption refraction light microscopy types compound bright field oil immersion microscopy dark field microscopy phase contrast dual beam phase contrast phase. Ppt presentation on viruses which is created by jeddy and available for free download viruses powerpoint presentation. The gram stain reaction and koh test results were compared with the antibiotic disk susceptibilities vancomycin and colistin the preliminary. Bacterial classification, structure and function introduction the purpose of this lecture is to introduce you to terminology used in microbiology. In the laboratory, the gram stain test is the primary test employed in ppt.
Differentiate bacteria into two large groups the gram positive and the gram negative. Free download viruses powerpoint presentation slides free. In addition this stain also allows determination of cell morphology, size, and arrangement. Download gram positive bacteria 319476 ppt for free. Flood airdried, heatfixed smear of cells for 1 minute with crystal violet staining reagent. Developed gram staining technique which is unique to bacterial cells due to their cell wall construction. Gram staining or gram s method is an emprical method of differentiating bacterial species into two large groups gram positive and gram negative based on the chemical and physical properties of their cell wall. Ppt the gram stain powerpoint presentation free to. Who developed gram stains hans christian gram danish physician 1884 original purpose was to discern the difference between two types of pnuemonia. The gram stain control slides are prepared from standard quality control organisms. Staphylococcus epidermidis a gram positive cocci found on the. Screening of the quality of the clinical specimens such as sputum that should contain many. The difference in result is due to the differences in the cell wall structure or composition of bacteria that causes the different in the reaction with the series of reagents in gram staining talaro, 2007.
Ppt gram staining powerpoint presentation free to download id. The gram stain reaction, named after the danish bacteriologist hans christian gram, was originally devised in 1882. Gram stain this is the most important staining method in bacteriology. A free powerpoint ppt presentation displayed as a flash slide show on id. Gonorrhea gonococcal urethritis gonococcal cervicitis gonorrhea gram stain of urethral discharge bartholins abscess bartholins abscess gonococcal ophthalmia disseminated gonorrhea skin lesion disseminated gonorrhea skin lesion gonorrhea gonococcal urethritis gonococcal cervicitis gonorrhea gram stain of urethral discharge bartholins abscess bartholins abscess gonococcal.
The gram stain procedure was originally developed by the danish physician hans. Differential stain distinguishes between different groups of bacteria based on their staining ability. Without it, dye is not capable of binding to and staining the tissue. Gram staining is a differential staining technique that differentiates bacteria into two groups. Find powerpoint presentations and slides using the power of, find free presentations research about capsule staining ppt. View and download powerpoint presentations on staining techniques ppt. Powerpoint is the worlds most popular presentation software which can let you create professional bacteria are everywhere powerpoint presentation easily and in no time. Mordants substance that causes certain staining reactions to take place by forming a link between the tissue and the stain. How it works gram positive bacteria have a thick layer ofpeptidoglycanup to 90%in their cell wall gram negative bacteria have a thinner layer 5 20%of this glycoprotein. Gram negative bacteria have a thin peptide layer and space surrounding it so the cell wall is.
Ppt the gram stain powerpoint presentation free to download id. He developed a new method to stain bacteria so they can be visible in specimen samples. Gram staining of bacterial cells is neither an accurate nor elaborate technique, but nevertheless it is practically useful to distinguish two big domains of eubacteria species. The most widely used staining procedure in microbiology is the gram stain, discovered by the danish scientist and physician hans christian joachim gram in 1884. Staining, therefore, is of primary importance for the. Bacteria may be stained by a variety of dyes, including. View and download powerpoint presentations on gram positive cocci ppt. Generally, an ebook can be downloaded in five minutes or less. The adobe flash plugin is needed to view this content. Khan academy is a nonprofit with the mission of providing a free, worldclass education for anyone, anywhere.
Overheating of smears when heat fixation is used d. Who developed gram stains hans christian gram danish physician 1884 original purpose was to discern the difference. Find powerpoint presentations and slides using the power of, find free presentations research about spore stain ppt. Introduction as bacteria consist of clear protoplasmic matter, differing but slightly in refractive index from the medium in which they are growing, it is difficult with the ordinary microscope, except when special methods of illumination are used, to set them in the unstained condition. The gram stain of a bacteria is determined by one thing gram positive gram negative the thickness of the petidoglycan in its cell wall materials background clothes pin 2. Gram negative bacteria release the first dye used and appear red from the second counter dye. Different bacteria stain differently to a common staining procedure. The staining technique, developed in the late 1700s by christian gram classifies the rigid cell walled bacteria into one of two groups based on whether they are able to resist the decolorizing action of an alcoholic. Gram stain control slide provides two airdried and methanolfixed droplets within two etched circles. Jan 08, 2020 the gram staining technique is the most important and widely used microbiological differential staining technique. Ppt gram stain procedure powerpoint presentation free. Gram positive bacteria that include most of the firmicutes, and gram negative bacteria that include the rest.
It includes introduction, history,principle, method of gram staining procedure slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Gram himself never used the red counterstaining in order to visualize the gram. Classic gram staining techniques involve the following steps. Ppt gram stain powerpoint presentation, free download. Gram staining is the property of its rightful owner. The microscopicanalysis using gram staining technique showed that majority of the bacterial isolates were grampositive bacteria while only three of. To examine whether gram staining can influence the choice of antibiotic for the treatment of peritonsillar abscess. View and download powerpoint presentations on capsule staining ppt. Biology ppts videos microbiology mcqs microbiology notes. The acid fast stain was discovered by ehrlich, who found that after staining with aniline dyes, tubercle bacilli resist decolourisation with acids. Gram staining is a differential staining technique that provides an easy differentiation of bacteria into one of two groups. Learning how to perform a gram stain will be the starting step in determining where to start when identifying bacteria for illnesses, disease or infection. Principle, procedure and results february 2, 2015 acharya tankeshwar bacteriology, staining techniques in microbiology 45 gram staining method, the most important procedure in microbiology, was developed by danish physician hans christian gram in 1884.
Beyer individual supplies microscope slide clothes pin inoculating loop or wood splint paper towels shared supplies crystal violet stain safranin red stain grams iodine ethyl alcohol denatured water bottle rinsing basin alcohol burner wax pencil matches microscope create a staining area on your microscope slide obtain a clean microscope slide draw a. Gram staining a important technique a staining technique used to classify bacteria. Scribd is the worlds largest social reading and publishing site. Crystal violet primary stain for 1 minrinse gram s iodine mordant for 1 minrinse acetonealcohol decolorizer for 5 seconds safranin counterstain for 1 minrinse gram negative red and gram positive blue assess gram reaction a. In the first step, the nuclei are stained with a hematoxylin solution. Actinomyces israelii gram stain gram positive bacteria that form long, branching filaments resembling the fungi 26.
Stain removal ppt free download as powerpoint presentation. Ppt gram staining powerpoint presentation, free download. Bacteria gram stain give what you know about genetics and molecular biology, describe another way you might be able to identify specific bacteria. Gram staining free download as powerpoint presentation. Find powerpoint presentations and slides using the power of, find free presentations research about gram staining ppt. Ppt gramstain powerpoint presentation free to download.
Pdf gram staining for the treatment of peritonsillar abscess. This helps you give your presentation on bacteria are everywhere in a conference, a school lecture, a business proposal, in a webinar and business and professional representations the uploader spent hisher valuable time to. Oral microbiology bacteria causing inflammations and abscesses. Gram staining chelsea bianchi how does gram staing work. Cover different classification schemes for grouping bacteria, especially the use of the gram stain 2. Learn for free about math, art, computer programming, economics, physics, chemistry, biology, medicine, finance, history, and more. Gonorrhea centers for disease control and prevention. The gram stain, the most widely used staining procedure in bacteriology, is a complex. The gram stain is almost always the first step in the identification of a bacterial organism, while gram staining is a valuable. Ppt giemsa powerpoint slides power point presentations. Times new roman arial wingdings default design slide 1 key words prokaryotes prokaryotes bacteria eukaryotes prokaryotic cell versus eukaryotic cell bacteria versus archaebacteria bacteria versus archaebacteria slide 9 bacteria slide 11 slide 12 slide outer membrane gram negative cell envelope gram positive cell envelope flagella slide. Find powerpoint presentations and slides using the power of, find free presentations research about gram positive cocci ppt.
The gram stain is the property of its rightful owner. Principle, procedure, interpretation, examples and animation last updated. Record sketches of the organisms, size, color, morphology, and culture identification. State why the gram stain is said to be a differential stain describe the differences between a gram positive and a gram negative cell wall state the mechanism of the gram stain and why differential staining of. Gram staining of bacteria the purpose of gram staining is to determine one characteristic property of bacteria. Gram staining 2 free download as powerpoint presentation. Differential stain hans christian gram, a danish doctor. Basic microbiology lab reporting gram staining free download as powerpoint presentation. Fix the dried film by passing it briefly through a bunsen flame. Label the frosted side of your slide with your initials, the name of the organism, and the date. Gram stain mohammad rahbar reporting 4 issue a written preliminary report with the gram s stain results 5 telephonically notify the ordering provider or ward of. This site uses cookies to deliver our services and to show you relevant ads and presentations.
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